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KMID : 0857020060210010310
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Kosin Medical Journal
2006 Volume.21 No. 1 p.310 ~ p.317
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The Effect of Combined Treatmens with Melatonin and Water-soluble Vitamin E in Lipid Peroxidation and Survival Rate after Paraquat Intoxication in Rats
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Kim Jin-Ha
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Abstract
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Objectives : Paraquat, an oxygen radical-generating agent, is a highly toxic compund for humans ans animals. This study was examined the effect of combined treatment of melatonin and Trolox^(®)(water-soluble analogue of α-tocopherol) in lipid peroxidation and mortality after paraquat intoxication in rats.
Methods : Paraquat(20mg/kg) was injected i.p. into Spraque-Dawely rats. After the administration of paraquat, rats were treated with melatonin(10mg/kg), Trolox^(®)(100mg/kg), combined melatonin and Trolox^(®). All rats were killed by decapitation 24hours after paraquat administration and then examined the concentration of malonaldehye and 4-hydroxyalkenal (MDA + 4-HAE) as indices of lipid peroxidation in rat lung and liver. In a survival study, continous i.p. infusion of melatonin (50mg/kg/day) for 14 days using Alzet^(®) Osmotic pump (2ML2) and Trolox^(®)(100mg/kg/day) was injected i.p. after paraquat (20mg/kg) poisoning into rats for 14days.
Results : Paraquat alone increased MDA + 4-HAE levels in lung, with this increase being reduced by melatonin and Trolox^(®), and also combined treatment with melatonin and Trolox^(®) completely reversed this effect. Paraquat at 20mg/kg did not induced any significant change in liver lipid peroxidation. In a result of survival analysis, percent survival was 100 % (8/8, control), 0 % (0/8, paraquat only), 62.5 % (5/8, paraquat + melatonin), 37.5 % (3/8, paraquat + Trolox^(®)), 75.0 % (6/8, paraquat + melatonin + Trolox^(®)) at 14 days after paraquat intoxication.
Conclusions : These results indicate that melatonin and Trolox^(®) protect lipid peroxidation and increase survival time in paraquat intoxicated rats. Combined treatment with melatonin and Trolox^(®) seems to have more survival benefit than single agent treatment in paraquat intoxication.
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KEYWORD
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Paraquat, melatonin, Trolox^(®), combied treatment, lipid peroxidation, survival time
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